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Journal of Clinical Microbiology, April 2008, p. 1252-1258, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02140-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Prevalence and Genetic Diversity of Aichi Virus Strains in Stool Samples from Community and Hospitalized Patients{triangledown}

K. Ambert-Balay,1* M. Lorrot,2 F. Bon,1 H. Giraudon,1 J. Kaplon,1 M. Wolfer,2 P. Lebon,3 D. Gendrel,2 and P. Pothier1

Laboratoire de Virologie, Reference Laboratory for Enteric Viruses, Centre Hospitalier Universitaire, and UFRs Médecine et Pharmacie, Dijon,1 Service de Pédiatrie, Centre Hospitalier Saint Vincent de Paul, Paris,2 Laboratoire de Virologie, Centre Hospitalier Saint Vincent de Paul, Paris, France3

Received 6 November 2006/ Returned for modification 14 January 2008/ Accepted 30 January 2008

Aichi virus has been proposed as a causative agent of gastroenteritis. A total of 457 stool specimens from children hospitalized with acute diarrhea and 566 stool specimens from adults and children involved in 110 gastroenteritis outbreaks were screened for the presence of Aichi virus by reverse transcription-PCR (RT-PCR) amplification of the genomic region of the 3C and 3D (3CD) nonstructural proteins. Our results show a low incidence of Aichi virus in pediatric samples and the existence of mixed infections with other microbiological agents in some cases. From the outbreak survey, it appears that the presence of Aichi virus is an indicator of mixed infections causing gastroenteritis outbreaks and that it could be involved in half of the oyster-associated outbreaks. A second RT-PCR was developed to amplify a part of the VP1 gene. The phylogenetic analysis showed a good correlation between the two classifications based on 3CD and VP1 gene sequences and revealed the prevalence of genotype A in France. It also allowed us to partially describe an Aichi virus strain that could represent a new genotype, thus suggesting the existence of a certain diversity.

* Corresponding author. Mailing address: Laboratoire de Virologie, CNR des Virus Entériques, CHU Dijon, 2 boulevard Maréchal de Lattre de Tassigny, BP 77908, 21079 Dijon, France. Phone: 33 380 293 437. Fax: 33 380 293 604. E-mail: katia.balay{at}chu-dijon.fr

{triangledown} Published ahead of print on 6 February 2008.

Journal of Clinical Microbiology, April 2008, p. 1252-1258, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02140-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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