This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, Y.
Right arrow Articles by Jiang, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Y.
Right arrow Articles by Jiang, Y.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2008, p. 1317-1321, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.00073-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Prevalence of Plasmid-Mediated AmpC β-Lactamases in a Chinese University Hospital from 2003 to 2005: First Report of CMY-2-Type AmpC β-Lactamase Resistance in China{triangledown}

Yi Li,1 Qing Li,1 Yuzhen Du,1 Xiaofei Jiang,2 Jin Tang,1 Jianqiang Wang,1 Guilan Li,1 and Yanqun Jiang1*

Department of Clinical Laboratory Medicine, Sixth Affiliate Hospital of Shanghai Jiao Tong University,1 Center of Laboratory Medicine of Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China2

Received 10 January 2007/ Returned for modification 5 June 2007/ Accepted 8 January 2008

The aim of this study was to investigate the prevalences of plasmid-mediated AmpC β-lactamases (PABLs) in isolates of Escherichia coli and Klebsiella spp. from a university hospital in China. A total of 1,935 consecutive nonrepeat clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca were collected between January 2003 and July 2005. The isolates with cefoxitin zone diameters less than 18 mm (screen positive) were selected for PCR of the blaAmpC genes and sequencing. Fifty-four (2.79%) isolates harbored PABLs, as demonstrated by PCR and isoelectric focusing. Sequence analysis revealed the presence of blaDHA-1 and blaCMY-2 genes. The Southern blot hybridization studies confirmed that blaCMY-2 and blaDHA-1 were located on plasmids. Based on species, PABLs were detected in 4.29% (29 isolates of DHA-1 and 1 isolate of CMY-2) of K. pneumoniae, 1.91% (11 isolates of DHA-1 and 12 isolates of CMY-2) of E. coli, and 3.03% (1 isolate of DHA-1) of K. oxytoca isolates. In contrast to our anticipation, the occurrence rate of DHA-1-producing K. pneumonia significantly decreased (P < 0.01), from 7.54% in 2003 to 2.72% in 2004. The results of random amplified polymorphic DNA analysis indicate that the prevalences of DHA-1-producing K. pneumoniae and CMY-2-producing E. coli strains were not due to epidemic strains. In conclusion, DHA-1 was the most prevalent acquired AmpC beta-lactamase in this collection of isolates from a medical center in China, and DHA-1-producing K. pneumoniae was the most prevalent bacterium harboring a PABL. To the best of our knowledge, this is the first report of CMY-2-type AmpC β-lactamases in the Chinese mainland.

* Corresponding author. Mailing address: Department of Clinical Laboratory Medicine, Sixth Affiliate Hospital of Shanghai Jiao Tong University, 600 Yi Shan Rd., Shanghai 200233, People's Republic of China. Phone: 86 021 64369181 8735. Fax: 86 021 64701361. E-mail: yqjiang25{at}yahoo.com

{triangledown} Published ahead of print on 27 February 2007.

Journal of Clinical Microbiology, April 2008, p. 1317-1321, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.00073-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jacoby, G. A. (2009). AmpC {beta}-Lactamases. Clin. Microbiol. Rev. 22: 161-182 [Abstract] [Full Text]  
  • Tan, T. Y., Ng, L. S. Y., He, J., Koh, T. H., Hsu, L. Y. (2009). Evaluation of Screening Methods To Detect Plasmid-Mediated AmpC in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. Antimicrob. Agents Chemother. 53: 146-149 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»