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Journal of Clinical Microbiology, April 2008, p. 1478-1484, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.01873-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Prophages in Listeria monocytogenes Contain Single-Nucleotide Polymorphisms That Differentiate Outbreak Clones within Epidemic Clones{triangledown}

Yi Chen* and Stephen J. Knabel

Department of Food Science, The Pennsylvania State University, University Park, Pennsylvania 16802

Received 20 September 2007/ Returned for modification 12 November 2007/ Accepted 28 January 2008

A fragment end ligation-mediated PCR strategy was used to analyze the AscI pulsed-field gel electrophoresis patterns of Listeria monocytogenes epidemic clone II (ECII), which led to the identification of single-nucleotide polymorphisms (SNPs) in prophage regions that differentiated the two ECII outbreak clones. SNPs in prophages that differentiated the outbreak clones of ECIII and -IV were also identified.

* Corresponding author. Mailing address: Center for Food Safety and Applied Nutrition, FDA, HFS-712, 5100 Paint Branch Parkway, College Park, MD 20740. Phone: (301) 436-2783. Fax: (301) 436-2644. E-mail: yi.chen{at}fda.hhs.gov

{triangledown} Published ahead of print on 6 February 2008.

Journal of Clinical Microbiology, April 2008, p. 1478-1484, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.01873-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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