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GeneXpert Enterovirus Assay: One-Year Experience in a Routine Laboratory Setting and Evaluation on Three Proficiency Panels

Katja Seme,¹ Tina Moilnik,¹ Kristina Fujs Komlo,¹ Ana Doplihar,¹ David H. Persing,² and Mario Poljak^1*

Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia,¹ Cepheid, Sunnyvale, California²

Received 25 August 2007/ Returned for modification 25 October 2007/ Accepted 25 January 2008

A prospective unblinded comparative evaluation of three assays for the detection of enteroviral RNA performed on 83 positive and 79 negative cerebrospinal fluid samples showed initial and resolved sensitivities of 90.4% and 98.8%, respectively, for the Cepheid GeneXpert enterovirus assay; 94.0% and 97.6%, respectively, for the Argene enterovirus consensus kit; and 100% and 100%, respectively, for an in-house real-time PCR. The initial and resolved specificities were 100% for all three assays.

Published ahead of print on 6 February 2008.