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Journal of Clinical Microbiology, April 2008, p. 1510-1513, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.01694-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

GeneXpert Enterovirus Assay: One-Year Experience in a Routine Laboratory Setting and Evaluation on Three Proficiency Panels{triangledown}

Katja Seme,1 Tina Mocilnik,1 Kristina Fujs Komlos,1 Ana Doplihar,1 David H. Persing,2 and Mario Poljak1*

Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia,1 Cepheid, Sunnyvale, California2

Received 25 August 2007/ Returned for modification 25 October 2007/ Accepted 25 January 2008

A prospective unblinded comparative evaluation of three assays for the detection of enteroviral RNA performed on 83 positive and 79 negative cerebrospinal fluid samples showed initial and resolved sensitivities of 90.4% and 98.8%, respectively, for the Cepheid GeneXpert enterovirus assay; 94.0% and 97.6%, respectively, for the Argene enterovirus consensus kit; and 100% and 100%, respectively, for an in-house real-time PCR. The initial and resolved specificities were 100% for all three assays.

* Corresponding author. Mailing address: Institute of Microbiology and Immunology, Faculty of Medicine, Zaloska 4, Ljubljana 1000, Slovenia. Phone: 386 1 543 7453. Fax: 386 1 543 7418. E-mail: mario.poljak{at}mf.uni-lj.si

{triangledown} Published ahead of print on 6 February 2008.

Journal of Clinical Microbiology, April 2008, p. 1510-1513, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.01694-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mahony, J. B. (2008). Detection of Respiratory Viruses by Molecular Methods. Clin. Microbiol. Rev. 21: 716-747 [Abstract] [Full Text]  


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