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Journal of Clinical Microbiology, April 2008, p. 1523-1525, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02100-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Is a Two-Step Glutamate Dehyrogenase Antigen-Cytotoxicity Neutralization Assay Algorithm Superior to the Premier Toxin A and B Enzyme Immunoassay for Laboratory Detection of Clostridium difficile?

Clinical Microbiology-Immunology Laboratories, University of North Carolina Hospitals, and Departments of Microbiology-Immunology and Pathology-Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27514

Received 31 October 2007/ Returned for modification 11 December 2007/ Accepted 25 January 2008

A two-step algorithm for the detection of Clostridium difficile by the use of C. Diff Quik Chek (TechLab, Blacksburg, VA) and a tissue culture cytotoxicity neutralization assay was found to be more sensitive than the widely used solid-phase enzyme immunoassay (EIA), the Premier toxin A and B EIA (Meridian Bioscience, Cincinnati, OH), and a newly developed, rapid single-test EIA for C. difficile toxins A and B (Tox A/B Quik Chek; TechLab).

Published ahead of print on 6 February 2008.