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Journal of Clinical Microbiology, April 2008, p. 1534-1536, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02234-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of Staphylococcus aureus and Determination of Methicillin Resistance Directly from Positive Blood Cultures by Isothermal Amplification and a Disposable Detection Device{triangledown}

James Goldmeyer,1 Haijing Li,2 Melinda McCormac,3 Steve Cook,4 Charles Stratton,2,3 Bertrand Lemieux,1 Huimin Kong,1 Wen Tang,1 and Yi-Wei Tang2,3*

BioHelix Corporation, Beverly, Massachusetts 01915,1 Departments of Medicine,2 Pathology, Vanderbilt University Medical Center, Nashville, Tennessee 37232,3 Lahey Clinic Medical Center, Burlington, Massachusetts 018054

Received 19 November 2007/ Returned for modification 4 January 2008/ Accepted 16 January 2008

A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively.

* Corresponding author. Mailing address: Molecular Infectious Disease Laboratory, Vanderbilt University Hospital, 4605 TVC, Nashville, TN 37232-5310. Phone: (615) 322-2035. Fax: (615) 343-8420. E-mail: yiwei.tang{at}vanderbilt.edu

{triangledown} Published ahead of print on 30 January 2008.

Journal of Clinical Microbiology, April 2008, p. 1534-1536, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02234-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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