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Journal of Clinical Microbiology, April 2008, p. 1537-1540, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.00030-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid and Accurate Identification of Candida albicans Isolates by Use of PNA FISH^Flow

AdvanDx, Inc., Woburn, Massachusetts,¹ The Johns Hopkins Medical Institutes, Baltimore, Maryland,² Columbia University Medical Center, New York, New York,³ Statens Serum Institut, Copenhagen, Denmark⁴

Received 7 January 2008/ Returned for modification 22 January 2008/ Accepted 6 February 2008

We developed the simple, rapid (1 h), and accurate PNA FISH^Flow method for the identification of Candida albicans. The method exploits unique in solution in situ hybridization conditions under which the cells are simultaneously fixed and hybridized. This method facilitates the accurate identification of clinical yeast isolates using two scoring techniques: flow cytometry and fluorescence microscopy.

Published ahead of print on 20 February 2008.