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Journal of Clinical Microbiology, May 2008, p. 1686-1691, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02068-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Dynamic Transmission of Numerous Anaplasma phagocytophilum Genotypes among Lambs in an Infected Sheep Flock in an Area of Anaplasmosis Endemicity{triangledown}

Georgia A. F. Ladbury,1 Snorre Stuen,2 Rachael Thomas,1 Kevin J. Bown,1 Zerai Woldehiwet,1 Erik G. Granquist,2 Karin Bergström,3 and Richard J. Birtles1*

Department of Veterinary Pathology, Faculty of Veterinary Science, University of Liverpool, Leahurst Campus, Chester High Rd., Neston, Cheshire CH64 7TE, United Kingdom,1 Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, Kyrkjevegen 332/334, N-4325 Sandnes, Norway,2 Department of Bacteriology, National Veterinary Institute, SE-751 89, Uppsala, Sweden3

Received 24 October 2007/ Returned for modification 25 November 2007/ Accepted 17 March 2008

The transmission dynamics of Anaplasma phagocytophilum strains circulating within juvenile members of a sheep flock grazing on an Ixodes ricinus-infested pasture in southern Norway were monitored. PCR-based detection of the bacterial p44 fragments in the blood of 16 lambs sampled weekly for 16 weeks following their release into pasture revealed rickettsemia in all animals, with an increasing proportion of infected animals as the survey progressed. Comparison of partial msp4 sequences obtained from infected blood samples revealed 24 distinct genotypes, some of which were repeatedly encountered, occurring in up to six sheep over a 14-week period, whereas others were observed only once. Individual sheep were infected by up to five distinct genotypes, with a specific genotype being encountered for between one and three consecutive weeks, and in some sheep, genotypes detected early in the study were also present in later samples. In general, detection of A. phagocytophilum by PCR correlated well with the observation of infected neutrophils in blood smears. Together these results reveal a previously unrecognized diversity of A. phagocytophilum strains simultaneously circulating within an infected population in an area of endemicity and are consistent with a remarkably dynamic transmission of strains among infected animals.

* Corresponding author. Mailing address: Department of Veterinary Pathology, Faculty of Veterinary Sciences, University of Liverpool, Leahurst Campus, Chester High Road, Cheshire CH64 7TE, United Kingdom. Phone: 44 151 794 6122. Fax: 44 151 794 6005. E-mail: rjbirt{at}liv.ac.uk

{triangledown} Published ahead of print on 26 March 2008.

Journal of Clinical Microbiology, May 2008, p. 1686-1691, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02068-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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