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Journal of Clinical Microbiology, May 2008, p. 1818-1823, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02255-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of New Staphylococcal Cassette Chromosome mec (SCCmec) and Topoisomerase Genes in Fluoroquinolone- and Methicillin-Resistant Staphylococcus pseudintermedius{triangledown}

Sybill Descloux, Alexandra Rossano, and Vincent Perreten*

Institute of Veterinary Bacteriology, University of Bern, CH-3001 Bern, Switzerland

Received 21 November 2007/ Returned for modification 13 January 2008/ Accepted 20 February 2008

Fluoroquinolone- and methicillin-resistant Staphylococcus pseudintermedius isolates harbor two new staphylococcal cassette chromosome mec (SCCmec) elements that belong to class A, allotype 3 (SCCmec II-III), and to the new allotype 5 (SCCmec VII). Analysis of the complete nucleotide sequences of the topoisomerase loci gyrB/gyrA and grlB/grlA revealed mutations involved in fluoroquinolone resistance.

* Corresponding author. Mailing address: Institute of Veterinary Bacteriology, University of Bern, Länggass-Strasse 122, Postfach, CH-3001 Bern, Switzerland. Phone: 41 31 631 2430. Fax: 41 31 631 2634. E-mail: vincent.perreten{at}vbi.unibe.ch

{triangledown} Published ahead of print on 27 February 2008.

Journal of Clinical Microbiology, May 2008, p. 1818-1823, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02255-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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