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Journal of Clinical Microbiology, May 2008, p. 1835-1839, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.00956-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Repetitive-Sequence-Based PCR Using the DiversiLab System for Identification of Aspergillus Species{triangledown}

Dewey Hansen,1 Mimi Healy,2 Kristy Reece,2 Cheryl Smith,2 and Gail L. Woods1,3,4*

Associated Regional and University Pathologists, Inc., Infectious Diseases Laboratory, Salt Lake City, Utah,1 Bacterial Barcodes, Inc., Athens, Georgia,2 Associated Regional and University Pathologists, Inc., Institute for Clinical and Experimental Pathology, Salt Lake City, Utah,3 University of Utah School of Medicine, Department of Pathology, Salt Lake City, Utah4

Received 8 May 2007/ Returned for modification 16 August 2007/ Accepted 11 March 2008

Repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system was investigated for identification of Aspergillus. Ninety-five clinical isolates, identified by conventional methods, and five ATCC strains were tested. Sequencing of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) was performed on 2 isolates with discrepant rep-PCR and conventional results and 15 randomly selected outlier isolates. One isolate not identified by sequencing was excluded from analysis. After resolving discrepancies, all but one A. glaucus strain had ≥85% similarity to one or more strains of the same Aspergillus species in the mold database, thereby providing accurate identification. No isolate was misidentified.

* Corresponding author. Present address: Pathology and Laboratory Medicine Service (LR/113), Central Arkansas Veterans Healthcare System, 4300 W. 7th St., Little Rock, AR 72205. Phone: (501) 257-6433. Fax: (501) 526-4621. E-mail: Gail.Woods{at}va.gov

{triangledown} Published ahead of print on 26 March 2008.

Journal of Clinical Microbiology, May 2008, p. 1835-1839, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.00956-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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