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Journal of Clinical Microbiology, May 2008, p. 1840-1841, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.01554-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of a Commercial Direct Fluorescent-Antibody Assay for Human Metapneumovirus in Respiratory Specimens{triangledown}

Donald C. Vinh,1 Diane Newby,1 Hugues Charest,2 and Jane McDonald1*

Department of Medical Microbiology, Clinical Virology Laboratory, McGill University Health Centre, Montreal, Quebec, Canada,1 Laboratoire de Santé Publique du Québec/Institut National de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Quebec, Canada2

Received 3 August 2007/ Returned for modification 29 January 2008/ Accepted 12 March 2008

The performance of a commercially manufactured direct fluorescent-antibody assay kit for human metapneumovirus was compared to that of reverse transcriptase PCR, the current "gold standard." The kit demonstrated a sensitivity of 95.2%, a specificity of 100%, and an accuracy of 98.9%.

* Corresponding author. Mailing address: The Montreal Children's Hospital, 2300 Tupper, C-1341 Montreal, Quebec, Canada H3H 1P3. Phone: (514) 412-4313. Fax: (514) 412-4354. E-mail: jane.mcdonald{at}muhc.mcgill.ca

{triangledown} Published ahead of print on 26 March 2008.

Journal of Clinical Microbiology, May 2008, p. 1840-1841, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.01554-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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