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Journal of Clinical Microbiology, May 2008, p. 1842-1843, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02329-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Optimum Diagnostic Assay and Clinical Specimen for Routine Rotavirus Surveillance {triangledown}

Lauren J. Stockman,1,2* Mary A. Staat,3 Michol Holloway,3 David I. Bernstein,3 Tara Kerin,1,2 Jennifer Hull,1,2 Eileen Yee,1 Jon Gentsch,1 and Umesh D. Parashar1

National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta,1 Atlanta Research and Education Foundation, Decatur, Georgia,2 Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio3

Received 4 December 2007/ Returned for modification 28 January 2008/ Accepted 3 March 2008

Rates of detection of rotavirus were compared by diagnostic assay and specimen type. For bulk stools, rates of detection by reverse transcriptase PCR (RT-PCR) and enzyme immunoassay (EIA) were similar, but 18% of healthy controls tested positive by RT-PCR. Testing of bulk stools by EIA appears to be optimum for rotavirus surveillance.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Rd., MS F-22, Atlanta, GA 30333. Phone: (770) 488-7683. Fax: (770) 488-7761. E-mail: Lstockman{at}cdc.gov

{triangledown} Published ahead of print on 19 March 2008.

Journal of Clinical Microbiology, May 2008, p. 1842-1843, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02329-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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