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Journal of Clinical Microbiology, May 2008, p. 1844-1846, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02426-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Inability of Real-Time Reverse Transcriptase PCR Assay To Detect Subtype H7 Avian Influenza Viruses Isolated from Wild Birds{triangledown}

Zheng Xing,1* Carol Cardona,2 Phuong Dao,2 Beate Crossley,4 Sharon Hietala,3,4 and Walter Boyce1,5

Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California 95616,1 Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, California 95616,2 Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, California 95616,3 California Animal Health and Food Safety Laboratory, Davis, California,4 Wildlife Health Center, Davis, California5

Received 17 December 2007/ Returned for modification 3 February 2008/ Accepted 16 February 2008

We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 influenza viruses in poultry, is not reliable for wild bird surveillance.

* Corresponding author. Mailing address: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616. Phone: (530) 754-5623. Fax: (530) 752-7563. E-mail: zxing{at}ucdavis.edu

{triangledown} Published ahead of print on 5 March 2008.

Journal of Clinical Microbiology, May 2008, p. 1844-1846, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02426-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cardona, C., Slemons, R., Perez, D. (2009). The prevention and control of avian influenza: The avian influenza coordinated agriculture project. Poult. Sci. 88: 837-841 [Abstract] [Full Text]  


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