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Journal of Clinical Microbiology, May 2008, p. 1867-1869, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.00139-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of 16S rRNA Gene-Based PCR Assays for Genus-Level Identification of Helicobacter Species{triangledown}

H. Moyaert,* F. Pasmans, R. Ducatelle, F. Haesebrouck, and M. Baele

Department of Pathology, Bacteriology, and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium

Received 24 January 2008/ Accepted 1 March 2008

The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 103 and 1 viable bacterial cell per reaction mixture.

* Corresponding author. Mailing address: Department of Pathology, Bacteriology, and Avian Diseases, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium. Phone: 32-9-2647434. Fax: 32-9-2647494. E-mail: Hilde.Moyaert{at}UGent.be

{triangledown} Published ahead of print on 12 March 2008.

Journal of Clinical Microbiology, May 2008, p. 1867-1869, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.00139-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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