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Journal of Clinical Microbiology, May 2008, p. 1870-1872, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02175-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

New and Sensitive Assay for Determining Pseudomonas aeruginosa Metallo-Beta-Lactamase Resistance to Imipenem{triangledown} ,{dagger}

Jingyuan Tan,1 Johann D. D. Pitout,2,4 and David S. Guttman3*

Department of Cell and Systems Biology, University of Toronto, Toronto, Canada,1 Departments of Pathology and Laboratory Medicine and of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada,2 Department of Microbiology, Calgary Laboratory Services, Calgary, Canada,4 Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Canada3

Received 9 November 2007/ Returned for modification 29 December 2007/ Accepted 3 March 2008

We present an imipenem lysate metallo-beta-lactamase (MBL) functional assay. This assay eliminates false-positive results due to the bactericidal effects of EDTA, can be performed with inexpensive reagents available in most laboratories, and is as accurate as the MBL Etest. It is appropriate for both high-accuracy screens and laboratories in developing countries with limited resources.

* Corresponding author. Mailing address: Centre for the Analysis of Genome Evolution & Function, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada. Phone: (416) 978-6865. Fax: (416) 978-5878. E-mail: david.guttman{at}utoronto.ca

{triangledown} Published ahead of print on 12 March 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, May 2008, p. 1870-1872, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02175-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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