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Journal of Clinical Microbiology, June 2008, p. 1941-1945, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00323-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Bovine Mastitis Associated with Prototheca blaschkeae{triangledown}

Sara Marques,1,2* Eliane Silva,1,2 Christine Kraft,3 Júlio Carvalheira,1,4 Arnaldo Videira,1,5 Volker A. R. Huss,3 and Gertrude Thompson1,2

Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Largo Prof. Abel Salazar, 2, 4099-003 Porto, Portugal,1 Unidade Multidisciplinar de Investigação Biomédica, Universidade do Porto, Largo Professor Abel Salazar, 2, 4099-003 Porto, Portugal,2 Department für Biologie—Molekulare Pflanzenphysiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, D-91058 Erlangen, Germany,3 Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Rua Padre Armando Quintas, 4485-661 Vairão, Portugal,4 Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal5

Received 15 February 2008/ Returned for modification 16 March 2008/ Accepted 13 April 2008

Bovine mastitis is an important and complex disease responsible for economic losses in the dairy industry. Biotype II strains of the green alga Prototheca zopfii can be involved, most often resulting in chronic mastitis of difficult treatment associated with reduced milk production. This type of infection is rare, but the number of reported cases is increasing worldwide. In order to determine the kind of species involved in mastitis by Prototheca in northwest Portugal, 41 Prototheca isolates were genetically characterized. The algae are part of Prototheca isolates that were collected during a 6-year period, isolated from the milk of 41 dairy cows in a total of 22 herds with a history of increasing somatic cell counts, mild clinical signs of udder infection, and unsuccessful response to the usual therapy. PCR amplification of the 18S ribosomal DNA (rDNA), amplified rDNA restriction analysis, and phylogenetic analyses of the 18S rDNA sequences were performed. Thirty-seven isolates were identified as P. zopfii var. hydrocarbonea and four as Prototheca blaschkeae. These data suggest a high incidence of P. zopfii var. hydrocarbonea mastitis in the region and demonstrate for the first time the involvement of P. blaschkeae with bovine mammary gland infections.

* Corresponding author. Mailing address: Laboratório de Doenças Infecciosas do ICBAS, ICAV, Rua Padre Armando Quintas, 4485-661 Vairão, Portugal. Phone: (351) 252 660 400. Fax: (351) 252 661 780. E-mail: saramarques{at}mail.icav.up.pt

{triangledown} Published ahead of print on 23 April 2008.

Journal of Clinical Microbiology, June 2008, p. 1941-1945, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00323-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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