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Journal of Clinical Microbiology, June 2008, p. 1965-1967, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.01900-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparison of Immunoblotting, Calculation of the Goldmann-Witmer Coefficient, and Real-Time PCR Using Aqueous Humor Samples for Diagnosis of Ocular Toxoplasmosis{triangledown} ,{dagger}

A. Fekkar,1,2,3* B. Bodaghi,4 F. Touafek,1 P. Le Hoang,4 D. Mazier,1,2,3 and L. Paris1

AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Service Parasitologie Mycologie, Paris, F-75013, France,1 Université Pierre et Marie Curie-Paris 6, UMR S511 Paris, F-75013, France,2 INSERM, U511, Paris, F-75013, France,3 AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Service Ophtalmologie, Paris, F-75013, France4

Received 24 September 2007/ Returned for modification 17 November 2007/ Accepted 24 March 2008

We compared three biological methods for the diagnosis of ocular toxoplasmosis (OT). Paired aqueous humor and serum samples from 34 patients with OT and from 76 patients with other ocular disorders were analyzed by three methods: immunoblotting or Western blotting (WB), the calculation of the Goldmann-Witmer coefficient (GWC), and PCR. WB and GWC each revealed the intraocular production of specific anti-Toxoplasma immunoglobulin G in 81% of samples (30 of 37). PCR detected toxoplasmic DNA in 38% of samples (13 of 34). Nine of the 13 PCR-positive patients were immunocompetent. Combining the techniques significantly improved the diagnostic sensitivity, to 92% for the GWC-WB combination, 90% for the WB-PCR combination, and 93% for the GWC-PCR combination. The combination of all three techniques improved the sensitivity to 97%.

* Corresponding author. Mailing address: AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Service Parasitologie Mycologie, Paris, F-75013, France. Phone: 33 611881154. Fax: 33 1 42 16 0165. E-mail: arnaud.fekkar{at}hotmail.fr

{triangledown} Published ahead of print on 9 April 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, June 2008, p. 1965-1967, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.01900-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Talabani, H., Asseraf, M., Yera, H., Delair, E., Ancelle, T., Thulliez, P., Brezin, A. P., Dupouy-Camet, J. (2009). Contributions of Immunoblotting, Real-Time PCR, and the Goldmann-Witmer Coefficient to Diagnosis of Atypical Toxoplasmic Retinochoroiditis. J. Clin. Microbiol. 47: 2131-2135 [Abstract] [Full Text]  


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