This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishiko, H.
Right arrow Articles by Yamazaki, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishiko, H.
Right arrow Articles by Yamazaki, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2008, p. 2002-2008, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.01835-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Novel Human Adenovirus Causing Nosocomial Epidemic Keratoconjunctivitis{triangledown}

Hiroaki Ishiko,1* Yasushi Shimada,1 Tsunetada Konno,1 Akio Hayashi,1 Takeshi Ohguchi,2 Yoshitsugu Tagawa,2 Koki Aoki,2 Shigeaki Ohno,2 and Shudo Yamazaki3

Host Defense Laboratory, Mitsubishi Chemical Medience Corporation, Tokyo 174-8555, Japan,1 Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan,2 AIDS Vaccine Development Association, Tokyo 169-0075, Japan3

Received 14 September 2007/ Returned for modification 30 November 2007/ Accepted 23 March 2008

In 2000, we encountered cases of nosocomial infections with epidemic keratoconjunctivitis (EKC) at a university hospital in Kobe, in the western part of Japan. Two human adenovirus (HAdV) strains, Kobe-H and Kobe-S, were isolated from patients with nosocomial EKC infection. They were untypeable by existing neutralizing antisera; however, the isolate was neutralized with homologous antisera. We then encountered several cases of EKC due to nosocomial infections in eye clinics in different parts of Japan. A total of 80 HAdVs were isolated from patients with EKC at eight different hospitals. The partial hexon gene sequences of the isolates were determined and compared to those of the prototype strains of 51 serotypes. All isolates had identical partial hexon nucleotide sequences. Phylogenetic analysis classified these isolates into species of HAdV-D. The isolates showed 93.9 to 96.7% nucleotide identity with HAdV-D prototype strains, while all 32 HAdV-D prototype strains ranged from 93.2 to 99.2% identity. The sequences of the loop 2 and fiber knob regions from the representative strain, Kobe-H, were dissimilar in all prototype strains of 51 serotypes. We believe that this virus is a novel serotype of HAdV that causes EKC.

* Corresponding author. Mailing address: Host Defense Laboratory, Mitsubishi Chemical Medience Corporation, Shimura 3-30-1, Itabashi-ku, Tokyo 174-8555, Japan. Phone: 81-3-5994-2340. Fax: 81-3-5994-2972. E-mail: Ishiko.Hiroaki{at}mp.medience.co.jp

{triangledown} Published ahead of print on 2 April 2008.

Journal of Clinical Microbiology, June 2008, p. 2002-2008, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.01835-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kaneko, H., Iida, T., Ishiko, H., Ohguchi, T., Ariga, T., Tagawa, Y., Aoki, K., Ohno, S., Suzutani, T. (2009). Analysis of the complete genome sequence of epidemic keratoconjunctivitis-related human adenovirus type 8, 19, 37 and a novel serotype. J. Gen. Virol. 90: 1471-1476 [Abstract] [Full Text]  
  • Aoki, K., Ishiko, H., Konno, T., Shimada, Y., Hayashi, A., Kaneko, H., Ohguchi, T., Tagawa, Y., Ohno, S., Yamazaki, S. (2008). Epidemic Keratoconjunctivitis Due to the Novel Hexon-Chimeric-Intermediate 22,37/H8 Human Adenovirus. J. Clin. Microbiol. 46: 3259-3269 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»