This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Preza, D.
Right arrow Articles by Paster, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Preza, D.
Right arrow Articles by Paster, B. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2008, p. 2015-2021, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.02411-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Bacterial Profiles of Root Caries in Elderly Patients{triangledown} ,{dagger}

Dorita Preza,1* Ingar Olsen,1 Jørn A. Aas,1 Tiril Willumsen,1 Bjørn Grinde,1,4 and Bruce J. Paster2,3

Institute of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway,1 Department of Molecular Genetics, The Forsyth Institute,2 Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts,3 Norwegian Institute of Public Health, Oslo, Norway4

Received 17 December 2007/ Returned for modification 12 February 2008/ Accepted 24 March 2008

Culture-based studies have shown that Streptococcus mutans and lactobacilli are associated with root caries (RC). The purpose of the present study was to assess the bacterial diversity of RC in elderly patients by use of culture-independent molecular techniques and to determine the associations of specific bacterial species or bacterial communities with healthy and carious roots. Plaque was collected from root surfaces of 10 control subjects with no RC and from 11 subjects with RC. The bacterial 16S rRNA genes from extracted DNA were PCR amplified, cloned, and sequenced to determine species identity. From a total of 3,544 clones, 245 predominant species or phylotypes were observed, representing eight bacterial phyla. The majority (54%) of the species detected have not yet been cultivated. Species of Selenomonas and Veillonella were common in all samples. The healthy microbiota included Fusobacterium nucleatum subsp. polymorphum, Leptotrichia spp., Selenomonas noxia, Streptococcus cristatus, and Kingella oralis. Lactobacilli were absent, S. mutans was present in one, and Actinomyces spp. were present in 50% of the controls. In contrast, the microbiota of the RC subjects was dominated by Actinomyces spp., lactobacilli, S. mutans, Enterococcus faecalis, Selenomonas sp. clone CS002, Atopobium and Olsenella spp., Prevotella multisaccharivorax, Pseudoramibacter alactolyticus, and Propionibacterium sp. strain FMA5. The bacterial profiles of RC showed considerable subject-to-subject variation, indicating that the microbial communities are more complex than previously presumed. The data suggest that putative etiological agents of RC include not only S. mutans, lactobacilli, and Actinomyces but also species of Atopobium, Olsenella, Pseudoramibacter, Propionibacterium, and Selenomonas.

* Corresponding author. Mailing address: Institute of Oral Biology, Faculty of Dentistry, University of Oslo, PO Box 1052 Blindern, 0316 Oslo, Norway. Phone: (47) 22840366. Fax: (47) 22840301. E-mail: doritap{at}odont.uio.no

{triangledown} Published ahead of print on 2 April 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, June 2008, p. 2015-2021, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.02411-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vartoukian, S. R., Palmer, R. M., Wade, W. G. (2009). Diversity and Morphology of Members of the Phylum "Synergistetes" in Periodontal Health and Disease. Appl. Environ. Microbiol. 75: 3777-3786 [Abstract] [Full Text]  
  • VLKOVA, B., CELEC, P. (2009). Does Enterococcus faecalis Contribute to Salivary Thiobarbituric Acid-reacting Substances?. In Vivo 23: 343-345 [Abstract] [Full Text]  
  • Keijser, B.J.F., Zaura, E., Huse, S.M., van der Vossen, J.M.B.M., Schuren, F.H.J., Montijn, R.C., ten Cate, J.M., Crielaard, W. (2008). Pyrosequencing analysis of the Oral Microflora of healthy adults. JDR 87: 1016-1020 [Abstract] [Full Text]  
  • Beighton, D., Gilbert, S. C., Clark, D., Mantzourani, M., al-Haboubi, M., Ali, F., Ransome, E., Hodson, N., Fenlon, M., Zoitopoulos, L., Gallagher, J. (2008). Isolation and Identification of Bifidobacteriaceae from Human Saliva. Appl. Environ. Microbiol. 74: 6457-6460 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»