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Journal of Clinical Microbiology, June 2008, p. 2070-2073, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00203-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genetic Differentiation of Escherichia coli O157:H7 Clades Associated with Human Disease by Real-Time PCR{triangledown}

James T. Riordan, Sandeep B. Viswanath, Shannon D. Manning, and Thomas S. Whittam*

Microbial Evolution Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824

Received 1 February 2008/ Returned for modification 10 March 2008/ Accepted 26 March 2008

The rapid and accurate identification of Escherichia coli O157:H7 strains is central to reducing the impact of outbreaks. A real-time PCR-based approach to differentiating major outbreak lineages of O157 with novel single-nucleotide polymorphisms is described. The utility of this method is in detection of hypervirulent strains in cases of clinical disease.

* Corresponding author. Mailing address: National Food Safety & Toxicology Center, 165 Food Safety & Toxicology Building, Michigan State University, East Lansing, MI 48824. Phone: (517) 884-2033. Fax: (517) 432-2310. E-mail: whittam{at}msu.edu

{triangledown} Published ahead of print on 9 April 2008.

Journal of Clinical Microbiology, June 2008, p. 2070-2073, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00203-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liu, K., Knabel, S. J., Dudley, E. G. (2009). rhs Genes Are Potential Markers for Multilocus Sequence Typing of Escherichia coli O157:H7 Strains. Appl. Environ. Microbiol. 75: 5853-5862 [Abstract] [Full Text]  
  • Ooka, T., Terajima, J., Kusumoto, M., Iguchi, A., Kurokawa, K., Ogura, Y., Asadulghani, M., Nakayama, K., Murase, K., Ohnishi, M., Iyoda, S., Watanabe, H., Hayashi, T. (2009). Development of a Multiplex PCR-Based Rapid Typing Method for Enterohemorrhagic Escherichia coli O157 Strains. J. Clin. Microbiol. 47: 2888-2894 [Abstract] [Full Text]  


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