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Journal of Clinical Microbiology, June 2008, p. 2092-2094, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.02001-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparison of DNA Pyrosequencing with Alternative Methods for Identification of Mycobacteria{triangledown}

Loree C. Heller,1* Michael Jones,2 and Ray H. Widen1

Esoteric Testing, Department of Pathology, Tampa General Hospital, P.O. Box 1289, Tampa, Florida 33601,1 Microbiology Laboratory, Sacred Heart Hospital, 5151 N. 9th Avenue, Pensacola, Florida 325042

Received 11 October 2007/ Returned for modification 27 December 2007/ Accepted 9 April 2008

Identification of mycobacterial clinical isolates by pyrosequencing within the hypervariable A region of the 16S rRNA gene was compared to other identification methods. For >90% of isolates, these identifications correlated to the level of complex or species. For identification of many mycobacteria, pyrosequencing offers an inexpensive alternative to traditional sequencing.

* Corresponding author. Mailing address: Esoteric Testing, Department of Pathology, Tampa General Hospital, P.O. Box 1289, Tampa, FL 33601. Phone: (813) 844-4677. Fax: (813) 844-1312. E-mail: lheller{at}tgh.org

{triangledown} Published ahead of print on 16 April 2008.

Journal of Clinical Microbiology, June 2008, p. 2092-2094, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.02001-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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