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Journal of Clinical Microbiology, June 2008, p. 2095-2098, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.02216-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Performance of Vitek 2 in Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa Isolates with Different Mechanisms of β-Lactam Resistance{triangledown}

Annarita Mazzariol,1 Marco Aldegheri,1 Marco Ligozzi,1 Giuliana Lo Cascio,2 Raffaella Koncan,1 and Roberta Fontana1*

Dipartimento di Patologia, Sezione di Microbiologia, Università di Verona,1 Servizio di Microbiologia, Immunologia e Virologia, Azienda Ospedaliera, Verona, Italy2

Received 16 November 2007/ Returned for modification 18 January 2008/ Accepted 11 April 2008

A total of 78 isolates of Pseudomonas aeruginosa grouped according to the phenotype for ceftazidime and imipenem susceptibility/resistance were used to assess the accuracy of the Vitek 2 system in antimicrobial susceptibility testing. Comparisons were made with a MIC gradient test for piperacillin-tazobactam, ceftazidime, aztreonam, imipenem, meropenem, gentamicin, and ciprofloxacin. For the total of 546 isolate-antimicrobial combinations tested, the category agreement was 83.6%, with 2.0, 1.6, and 12.8% very major, major, and minor errors, respectively. Vitek 2 accuracy was influenced differently by the mechanism responsible for resistance, and interpretation of the results in relation to phenotype could improve the performance of the system.


* Corresponding author. Mailing address: Professor of Microbiology, Director of Clinical Microbiology Laboratory, Strada Le Grazie, 8, 37134 Verona, Italy. Phone: 39 45 8027191. E-mail: roberta.fontana{at}univr.it

{triangledown} Published ahead of print on 23 April 2008.


Journal of Clinical Microbiology, June 2008, p. 2095-2098, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.02216-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.







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