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Journal of Clinical Microbiology, June 2008, p. 2109-2111, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00043-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of Three Automated Nucleic Acid Amplification Systems for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in First-Void Urine Specimens{triangledown}

P. N. Levett,* K. Brandt, K. Olenius, C. Brown, K. Montgomery, and G. B. Horsman

Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada

Received 8 January 2008/ Returned for modification 16 February 2008/ Accepted 2 April 2008

A total of 500 first-void urine specimens were tested for the presence of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids using ProbeTec ET reagents on a Viper platform (BD Diagnostics, Mississauga, Ontario, Canada), Aptima Combo 2 reagents on a Tigris platform (Gen-Probe, Inc., San Diego, CA), and Abbott RealTime CT/NG reagents on an m2000 platform (Abbott Molecular Diagnostics, Des Plaines, IL). The performance of the three assays for detection of N. gonorrhoeae was comparable, but detection of C. trachomatis by the three assays showed more variation. All three platforms were suitable for the detection of C. trachomatis and N. gonorrhoeae, but additional factors, such as maximum daily specimen throughput, are important in evaluating automated systems for C. trachomatis and N. gonorrhoeae detection in high-volume laboratories.

* Corresponding author. Mailing address: Saskatchewan Disease Control Laboratory, 3211 Albert Street, Regina, Saskatchewan S4S 5W6, Canada. Phone: (306) 787-3135. Fax: (306) 787-1525. E-mail: plevett{at}health.gov.sk.ca

{triangledown} Published ahead of print on 9 April 2008.

Journal of Clinical Microbiology, June 2008, p. 2109-2111, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00043-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ho, M K, Lo, J Y C, Lo, A C T, Cheng, F K, Chan, F K (2009). Evaluation of replacing the existing diagnostic strategy for Neisseria gonorrhoeae and Chlamydia trachomatis infections with sole molecular testing of urine specimens in a sexually transmitted infection clinic setting. Sex. Transm. Infect. 85: 322-325 [Abstract] [Full Text]  


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