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Journal of Clinical Microbiology, June 2008, p. 2122-2124, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00122-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

In Vitro Replication of Varicella-Zoster Virus in Human Retinal Pigment Epithelial Cells{triangledown}

Jonas Schmidt-Chanasit,1,2,{dagger} Karoline Bleymehl,1,{dagger} Holger F. Rabenau,1 Rainer G. Ulrich,3 Jindrich Cinatl Jr.,1 and Hans Wilhelm Doerr1*

Institute of Medical Virology, Hospital of Johann Wolfgang Goethe University, D-60596 Frankfurt am Main, Germany,1 Bernhard Nocht Institute for Tropical Medicine, Diagnostic Virology Laboratory, D-20359 Hamburg, Germany,2 Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute for Novel and Emerging Infectious Diseases, D-17493 Greifswald, Germany3

Received 21 January 2008/ Returned for modification 17 February 2008/ Accepted 31 March 2008

Here we describe for the first time the productive in vitro infection of human retinal pigment epithelial cells by varicella-zoster virus (VZV), resulting in a typical cytopathic effect (CPE) that is characterized by enlarged cells with increased granularity. Depending on the CPE dissemination, high titers of up to 1.6 x 106 PFU of cell-free and cryostable VZV/ml can be recovered.

* Corresponding author. Mailing address: Institute of Medical Virology, Paul-Ehrlich-Strasse 40, 60596 Frankfurt am Main, Germany. Phone: 49-69-6301-5219. Fax: 49-69-6301-6477. E-mail: H.W.Doerr{at}em.uni-frankfurt.de

{triangledown} Published ahead of print on 9 April 2008.

{dagger} J.S.-C. and K.B. contributed equally to this study.

Journal of Clinical Microbiology, June 2008, p. 2122-2124, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00122-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Michaelis, M., Geiler, J., Klassert, D., Doerr, H. W., Cinatl, J. Jr (2009). Infection of Human Retinal Pigment Epithelial Cells with Influenza A Viruses. IOVS 50: 5419-5425 [Abstract] [Full Text]  
  • Milikan, J. C. M., Baarsma, G. S., Kuijpers, R. W. A. M., Osterhaus, A. D. M. E., Verjans, G. M. G. M. (2009). Human Ocular-Derived Virus-Specific CD4+ T Cells Control Varicella Zoster Virus Replication in Human Retinal Pigment Epithelial Cells. IOVS 50: 743-751 [Abstract] [Full Text]  


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