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Journal of Clinical Microbiology, June 2008, p. 2133-2136, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.01512-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization

Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany,¹ Federal Institute for Risk Assessment, Berlin, Germany²

Received 28 July 2007/ Returned for modification 26 November 2007/ Accepted 21 March 2008

Thermotolerant Campylobacter spp. (Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis) are leading causes of food-borne diarrhea in humans. In this study, the usefulness of fluorescence in situ hybridization (FISH) for the identification of Campylobacter isolates was investigated. A hierarchical FISH probe set that included six group-, genus-, and species-specific probes was developed and evaluated with 12 reference strains and 94 clinical isolates of Campylobacter, Arcobacter, and Helicobacter. FISH correctly identified all isolates to the genus level and detected all thermotolerant Campylobacter isolates. The assay showed high degrees of sensitivity for the identification of C. jejuni (90%), C. coli (97%), C. lari (81%), and C. upsaliensis (100%) to the species level.

Published ahead of print on 2 April 2008.