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Journal of Clinical Microbiology, July 2008, p. 2291-2297, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00239-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Are Variable-Number Tandem Repeats Appropriate for Genotyping Mycobacterium leprae?{triangledown}

Marc Monot,1,{ddagger} Nadine Honoré,1,{ddagger} Charlotte Balière,1 Baohong Ji,2 Samba Sow,3 Patrick J. Brennan,4 and Stewart T. Cole1,5*

Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France,1 Bactériologie-Hygiène, Faculté de Médecine Pitié-Salpêtrière, Paris, France,2 Centre National d'Appui à la Lutte Contre la Maladie, Bamako, Mali,3 Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682,4 Global Health Institute, EPFL, Lausanne, Switzerland5

Received 5 February 2008/ Returned for modification 11 April 2008/ Accepted 13 May 2008

Comparative genomics analysis of the Tamil Nadu strain of Mycobacterium leprae has uncovered several polymorphic sites with potential as epidemiological tools. In this study we compared the stability of two different markers of genomic biodiversity of M. leprae in several biopsy samples isolated from the same leprosy patient. The first type comprises five different variable-number tandem repeats (VNTR), while the second is composed of three single nucleotide polymorphisms (SNP). Contrasting results were obtained, since no variation was seen in the SNP profiles of M. leprae from 42 patients from 7 different locations in Mali whereas the VNTR profiles varied considerably. Furthermore, since variation in the VNTR pattern was seen not only between different isolates of M. leprae but also between biopsy samples from the same patient, these VNTR may be too dynamic for use as epidemiological markers for leprosy.

* Corresponding author. Mailing address: Global Health Institute, EPFL, Station 15, CH-1015 Lausanne, Switzerland. Phone: 41 21 693 18 51. Fax: 41 21 693 17 90. E-mail: stewart.cole{at}epfl.ch

{triangledown} Published ahead of print on 21 May 2008.

{ddagger} These authors contributed equally.

Journal of Clinical Microbiology, July 2008, p. 2291-2297, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00239-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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