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Journal of Clinical Microbiology, July 2008, p. 2377-2380, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00230-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Optimization of a Laboratory-Developed Test Utilizing Roche Analyte-Specific Reagents for Detection of Staphylococcus aureus, Methicillin-Resistant S. aureus, and Vancomycin-Resistant Enterococcus Species{triangledown}

Maitry S. Mehta,1 Suzanne M. Paule,1 Donna M. Hacek,1 Richard B. Thomson,1,2 Karen L. Kaul,1,2 and Lance R. Peterson1,2*

Evanston Northwestern Healthcare, Evanston, Illinois,1 Northwestern University's Feinberg School of Medicine, Chicago, Illinois2

Received 4 February 2008/ Returned for modification 21 March 2008/ Accepted 24 April 2008

Nasal and perianal swab specimens were tested for detection of Staphylococcus aureus and vancomycin-resistant Enterococcus species (VRE) using a laboratory-developed real-time PCR test and microbiological cultures. The real-time PCR and culture results for S. aureus were similar. PCR had adequate sensitivity, but culture was more specific for the detection of VRE.

* Corresponding author. Mailing address: Evanston Northwestern Healthcare, Department of Pathology and Laboratory Medicine, Clinical Microbiology Laboratory, Evanston Northwestern Healthcare, 2650 Ridge Avenue, Evanston, IL 60201. Phone: (847) 570-1637. Fax: (847) 733-5093. E-mail: lancer{at}northwestern.edu

{triangledown} Published ahead of print on 30 April 2008.

Journal of Clinical Microbiology, July 2008, p. 2377-2380, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00230-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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