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Journal of Clinical Microbiology, July 2008, p. 2423-2427, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00420-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Practical Method for Detection and Identification of Candida, Aspergillus, and Scedosporium spp. by Use of Rolling-Circle Amplification{triangledown}

Xiaoyong Zhou,1,{dagger} Fanrong Kong,2,{dagger} Tania C. Sorrell,2 Hui Wang,1 Yiqun Duan,1 and Sharon C. A. Chen2*

Research Laboratory for Infectious Skin Diseases, Department of Dermatology, Wuhan First Hospital, Wuhan 430022, People's Republic of China,1 Centre for Infectious Diseases and Microbiology, Westmead Hospital and the University of Sydney, Westmead, New South Wales, Australia2

Received 3 March 2008/ Returned for modification 2 April 2008/ Accepted 12 May 2008

A sensitive rolling-circle amplification (RCA)-based method utilizing species-specific padlock probes targeted to the internal transcribed spacer 2 region of the fungal ribosomal DNA gene complex was developed. The assay was rapid (2 hours) and specific. Of 28 fungal isolates (16 of Candida, six of Aspergillus, and six of Scedosporium spp.), all were all identified correctly.

* Corresponding author. Mailing address: Centre for Infectious Diseases and Microbiology, Westmead Hospital, Darcy Road, Westmead, New South Wales 2145, Australia. Phone: 61-2 9845 6255. Fax: 61-2 9893 8659. E-mail: sharon.chen{at}swahs.health.nsw.gov.au

{triangledown} Published ahead of print on 21 May 2008.

{dagger} These authors contributed equally to the work.

Journal of Clinical Microbiology, July 2008, p. 2423-2427, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00420-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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