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Journal of Clinical Microbiology, August 2008, p. 2681-2685, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00449-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Multicenter Evaluation of the New Vitek 2 Neisseria-Haemophilus Identification Card{triangledown}

Robert P. Rennie,1* Cheryl Brosnikoff,1 Sandy Shokoples,1 L. Barth Reller,2 Stanley Mirrett,2 William Janda,3 Kathy Ristow,3 and Ann Krilcich3

Medical Microbiology Laboratory, University of Alberta Hospital, Edmonton, AB, Canada,1 Clinical Microbiology Laboratory, Duke University Hospital, Durham, North Carolina,2 University of Illinois Medical Center, Chicago, Illinois3

Received 6 March 2008/ Returned for modification 16 June 2008/ Accepted 18 June 2008

The new Neisseria-Haemophilus identification (NH) card for Vitek 2 was compared with 16S rRNA gene sequencing (16S) as the reference method for accurate identification of Neisseria spp., Haemophilus spp., and other fastidious gram-negative bacteria. Testing was performed on the Vitek 2 XL system with modified software at three clinical trial laboratories. Reproducibility was determined with nine ATCC quality control strains tested 20 times over a minimum of 10 days at all three sites. A challenge set of 30 strains with known identifications and 371 recent fresh and frozen clinical isolates were also tested. Expected positive and negative biochemical reactions were also evaluated for substrate reproducibility. All microorganisms were tested on the NH card, and all clinical and stock isolates were saved for 16S testing. All reproducibility tests yielded expected results within a 95% confidence interval. For challenge microorganisms, there was 98% overall correct identification, including 8% low discrimination, 2% incorrect identification, and 0% unidentified. For clinical strains, there was 96.5% overall correct identification, including 10.2% low discrimination, 2.7% incorrect identification, and 0.8% unidentified. The 2.7% (10/371) of clinical isolates that gave an incorrect identification consisted of 7 isolates correct to genus and 3 strains incorrect to genus. There were an additional 27 strains (primarily Neisseria species) for which the 16S identification result was different from the NH card result. These were all unclaimed species by the system. The new NH card met all performance criteria within a 95% confidence interval compared to identification of clinical isolates by 16S.

* Corresponding author. Mailing address: Medical Microbiology (Dept. of Laboratory Medicine and Pathology), University of Alberta Hospital, WMC 4B1.19 8440-112 Street, Edmonton, AB, Canada T6G 2B7. Phone: (780) 407-7242. Fax: (780) 407-8599. E-mail: robertrennie{at}capitalhealth.ca

{triangledown} Published ahead of print on 25 June 2008.

Journal of Clinical Microbiology, August 2008, p. 2681-2685, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00449-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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