This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jeronimo, J.
Right arrow Articles by Wang, S. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jeronimo, J.
Right arrow Articles by Wang, S. S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2008, p. 2759-2765, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00188-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of Linear Array Human Papillomavirus Genotyping Using Automatic Optical Imaging Software{triangledown}

J. Jeronimo,1* N. Wentzensen,1 R. Long,2 M. Schiffman,1 S. T. Dunn,3 R. A. Allen,3 J. L. Walker,4 M. A. Gold,4 R. E. Zuna,3 M. E. Sherman,1 S. Wacholder,1 and S. S. Wang1

Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland,1 Communications Engineering Branch, National Library of Medicine, Bethesda, Maryland,2 Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Olahoma,3 Department of Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma4

Received 29 January 2008/ Returned for modification 24 March 2008/ Accepted 2 June 2008

Variations in biological behavior suggest that each carcinogenic human papillomavirus (HPV) type should be considered individually in etiologic studies. HPV genotyping assays might have clinical applications if they are approved for use by the FDA. A widely used genotyping assay is the Roche Linear Array HPV genotyping test (LA). We used LA to genotype the HPV isolates from cervical specimens from women with the full spectrum of cervical disease: cervical cancer, cervical intraepithelial neoplasia (CIN), and HPV infections. To explore the feasibility and value of the automated reading of the LA results, we custom-designed novel, optical imaging software that provides optical density measurements of LA bands. We compared unmagnified visual examination with the automated measurements. The two measurements were highly associated. By either method, the threshold between a negative and a positive result was fairly sharp, with a clear bimodal distribution. Visually, most positive results were judged to be strong or medium, with fewer equivocal results categorized as weak (9.5% of positive samples), very weak (6.5% of positive samples), or extremely weak (7.7% of positive samples). The automated measurements of the intensities were significantly associated with the strength of the visual categories (P < 0.001). At the extremes of the automated signal intensities (≤20 units or ≥120 units), the bands were almost always categorized visually as negative and positive, respectively. In the equivocal zone (20 to 119 units), specimens were more increasingly likely to be judged to be visually positive as the number of other, definite infections on the same strip increased (P for trend < 0.001). Multiple, concurrent infections comprise ≥25% of HPV infections; thus, any systematic visual tendency that influences their evaluation when the result is equivocal should be minimized. Therefore, automated reading is probably worth development if easy-to-calibrate hardware and software can be optimized.

* Corresponding author. Present address: PATH, 1455 NW Leary Way, Seattle, WA 98107. Phone: (206) 788-2413. Fax: (206) 285-6619. E-mail: jjeronimo{at}path.org

{triangledown} Published ahead of print on 11 June 2008.

Journal of Clinical Microbiology, August 2008, p. 2759-2765, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00188-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stevens, M. P., Garland, S. M., Tabrizi, S. N. (2008). Validation of an Automated Detection Platform for Use with the Roche Linear Array Human Papillomavirus Genotyping Test. J. Clin. Microbiol. 46: 3813-3816 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»