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Journal of Clinical Microbiology, September 2008, p. 3080-3083, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00979-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of KPC-2 in a Clinical Isolate of Proteus mirabilis and First Reported Description of Carbapenemase Resistance Caused by a KPC β-Lactamase in P. mirabilis{triangledown}

R. Tibbetts,1,{dagger} J. G. Frye,2 J. Marschall,3 D. Warren,3 and W. Dunne1*

Department of Pathology and Immunology, Division of Laboratory Medicine, Washington University School of Medicine, St. Louis, Missouri 63110,1 Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA, Agricultural Research Service, Athens, Georgia 30605,2 Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri3

Received 21 May 2008/ Returned for modification 16 June 2008/ Accepted 3 July 2008

An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the blaKPC carbapenemase gene produced an amplicon of the expected size which was confirmed to be blaKPC-2 by sequence analysis. Transformation of a susceptible Escherichia coli host with plasmid preparations from the isolate generated a transformant for which the MICs of all of the carbapenems tested were increased three- to fourfold. We believe this to be the first report of carbapenem resistance in P. mirabilis caused by the acquisition of blaKPC.

* Corresponding author. Mailing address: Department of Pathology and Immunology, Division of Laboratory Medicine, Washington University School of Medicine, St. Louis, MO 63122. Phone: (314) 362-1547. Fax: (314) 362-1461. E-mail: dunne{at}wustl.edu

{triangledown} Published ahead of print on 16 July 2008.

{dagger} Present address: Department of Pathology, Henry Ford Health System, 2799 West Grand Blvd., Detroit, MI 48202.

Journal of Clinical Microbiology, September 2008, p. 3080-3083, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00979-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bennett, J. W., Herrera, M. L., Lewis, J. S. II, Wickes, B. W., Jorgensen, J. H. (2009). KPC-2-Producing Enterobacter cloacae and Pseudomonas putida Coinfection in a Liver Transplant Recipient. Antimicrob. Agents Chemother. 53: 292-294 [Abstract] [Full Text]  
  • Marschall, J., Tibbetts, R. J., Dunne, W. M. Jr., Frye, J. G., Fraser, V. J., Warren, D. K. (2009). Presence of the KPC Carbapenemase Gene in Enterobacteriaceae Causing Bacteremia and Its Correlation with In Vitro Carbapenem Susceptibility. J. Clin. Microbiol. 47: 239-241 [Abstract] [Full Text]  


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