This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tavares, A.
Right arrow Articles by Couto, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tavares, A.
Right arrow Articles by Couto, I.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2008, p. 3097-3100, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00910-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Use of Fluorescence In Situ Hybridization for Rapid Identification of Staphylococci in Blood Culture Samples Collected in a Portuguese Hospital {triangledown} ,{dagger}

Ana Tavares,1,2,{ddagger} João Inácio,2 José Melo-Cristino,3 and Isabel Couto1,2*

UEI Micobactérias, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, Rua da Junqueira, nr. 96, 1349-008 Lisbon, Portugal,1 CREM, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Monte de Caparica, Portugal,2 Hospital de Santa Maria, Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Egas Moniz, 1649-028 Lisbon, Portugal3

Received 12 May 2008/ Returned for modification 28 May 2008/ Accepted 10 June 2008

Fluorescence in situ hybridization was used for the direct identification of staphylococci in blood cultures collected at a Portuguese hospital where staphylococci account for up to 35% of clinically relevant blood cultures. The assay was able to detect the presence/absence of staphylococci and distinguish Staphylococcus aureus from coagulase-negative staphylococci in 4.5 h.

* Corresponding author. Mailing address: UEI Micobactérias, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), Rua da Junqueira, nr. 96, 1349-008 Lisbon, Portugal. Phone: 351-21-3652652. Fax: 351-21-3632105. E-mail: icouto{at}ihmt.unl.pt

{triangledown} Published ahead of print on 18 June 2008.

{dagger} We dedicate this article to the memory of Prof. Isabel Spencer-Martins (1951-2008).

{ddagger} Present address: Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, ITQB, Universidade Nova de Lisboa, Oeiras, Portugal.

Journal of Clinical Microbiology, September 2008, p. 3097-3100, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00910-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»