This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Podzorski, R. P.
Right arrow Articles by Tang, Y.-W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Podzorski, R. P.
Right arrow Articles by Tang, Y.-W.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2008, p. 3107-3109, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.02347-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens{triangledown}

Raymond P. Podzorski,1* Haijing Li,3 Jian Han,2 and Yi-Wei Tang3,4

Department of Pathology, Waukesha Memorial Hospital, Waukesha, Wisconsin 53188,1 Genaco Biomedical Products, Inc., Huntsville, Alabama 35805,2 Departments of Pathology,3 Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 372324

Received 6 December 2007/ Returned for modification 14 March 2008/ Accepted 3 July 2008

We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant Staphylococcus aureus (MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the "gold standard," the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% (P = 0.002) and specificities of 95.8% and 98.6% (P < 0.05), respectively.

* Corresponding author. Mailing address: Department of Pathology, Waukesha Memorial Hospital, 725 American Ave., Waukesha, WI 53188. Phone: (262) 928-7635. Fax: (262) 928-7962. E-mail: raymond.podzorski{at}phci.org

{triangledown} Published ahead of print on 9 July 2008.

Journal of Clinical Microbiology, September 2008, p. 3107-3109, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.02347-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»