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Journal of Clinical Microbiology, September 2008, p. 3169-3172, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00052-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

CASE REPORT

Molecular Diagnosis of Saksenaea vasiformis Cutaneous Infection after Scorpion Sting in an Immunocompetent Adolescent{triangledown}

Pauline Lechevalier,1,2 Dea Garcia Hermoso,3 Agnes Carol,2 Stephane Bonacorsi,2 Latifa Ferkdadji,4 Franck Fitoussi,5 Olivier Lortholary,3,6 Antoine Bourrillon,1 Albert Faye,1 Eric Dannaoui,3,7 and Francois Angoulvant1*

AP-HP, Hôpital Robert Debré, Service de Pédiatrie Générale, Université Paris Diderot-Paris VII, Paris, France,1 AP-HP, Hôpital Robert Debré, Laboratoire de Microbiologie, Université Paris Diderot-Paris VII, Paris, France,2 Centre National de Référence Mycologie et Antifongiques, Unité de Mycologie Moléculaire, CNRS URA3012, Institut Pasteur, Paris, France,3 AP-HP, Hôpital Robert Debré, Service d'Anatomopathologie, Université Paris Diderot-Paris VII, Paris, France,4 AP-HP, Hôpital Robert Debré, Service de Chirurgie Orthopédique, Université Paris Diderot-Paris VII, Paris, France,5 AP-HP, Hôpital Necker-Enfants malades, Centre d'Infectiologie Necker-Pasteur, Université Paris Descartes, Paris, France,6 AP-HP, Hôpital Georges Pompidou, Unité de Parasitologie-Mycologie, Université Paris Descartes, Paris, France7

Received 10 January 2008/ Returned for modification 21 February 2008/ Accepted 3 July 2008

We report the first case of cutaneous mucormycosis after a scorpion sting in Tunisia. Histopathology showed broad aseptate hyphae suggestive of a Zygomycete. Saksenaea vasiformis was identified by PCR amplification and sequencing of the fungal DNA on a cutaneous biopsy. Successful treatment was obtained by surgery and liposomal amphotericin B.

* Corresponding author. Mailing address: Service de Pédiatrie générale, Hôpital Robert Debré, AP-HP, 48 Boulevard Sérurier, 75019 Paris, France. Phone: 33 1 40 03 53 61. Fax: 33 1 40 03 47 45. E-mail: francois.angoulvant{at}rdb.aphp.fr

{triangledown} Published ahead of print on 16 July 2008.

Journal of Clinical Microbiology, September 2008, p. 3169-3172, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00052-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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