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Journal of Clinical Microbiology, January 2009, p. 227-229, Vol. 47, No. 1
0095-1137/09/$08.00+0     doi:10.1128/JCM.01518-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Effect of ThinPrep Preparation on Human papillomavirus Detection and Genotyping in Rectal Samples by PCR{triangledown}

Sepehr N. Tabrizi,1,2* Matthew P. Stevens,1 Marina T. van Leeuwen,3 Claire M. Vajdic,3,4 Leon P. Botes,3 Gabriele Medley,5 Richard Hillman,6 Andrew Grulich,3 and Suzanne M. Garland1,2

Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia,1 Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Australia,2 National Centre in HIV Epidemiology & Clinical Research, University of New South Wales, Sydney, Australia,3 UNSW Cancer Research Centre, Prince of Wales Clinical School, University of New South Wales, Sydney, Australia,4 Melbourne Pathology, Melbourne, Australia,5 STI Research Centre, University of Sydney, Sydney, Australia6

Received 7 August 2008/ Returned for modification 22 September 2008/ Accepted 1 November 2008

Specimen-to-specimen carryover during ThinPrep slide preparation was evaluated by comparing human papillomavirus genotypes detected prior and subsequent to the ThinPrep processing of 121 PreservCyt samples. Overall, 52 samples generated concordant genotypes and 38 had additional and 21 had fewer genotypes postprocessing. PreservCyt samples should be aliquoted for PCR testing prior to ThinPrep processing.

* Corresponding author. Mailing address: The Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia. Phone: (613) 9344-2050. Fax: (613) 9344-2713. E-mail: sepehr.tabrizi{at}rch.org.au

{triangledown} Published ahead of print on 12 November 2008.

Journal of Clinical Microbiology, January 2009, p. 227-229, Vol. 47, No. 1
0095-1137/09/$08.00+0     doi:10.1128/JCM.01518-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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