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Journal of Clinical Microbiology, October 2009, p. 3082-3090, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00282-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genomic Analyses of Recombinant Adenovirus Type 11a in China{triangledown}

Zhaohui Yang,1,2,{dagger} Zhen Zhu,1,{dagger} Liuying Tang,1 Li Wang,3 Xiaojuan Tan,1 Pengbo Yu,3 Yong Zhang,1 Xiaoping Tian,3 Jingjun Wang,3 Yan Zhang,1 Dexin Li,1 and Wenbo Xu1*

State Key Laboratory for Molecular Virology & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Nanwei Road 27, Xuanwu District, Beijing 100050, People's Republic of China,1 Lanzhou University Second Hospital, Cuiyingmen Road 82, Chengguan District, Lanzhou 730000, People's Republic of China,2 Shaanxi Center for Disease Control and Prevention, Jiandong Road 3, Xian 710054, People's Republic of China3

Received 8 February 2009/ Returned for modification 30 March 2009/ Accepted 31 July 2009

Whole-genome sequencing of human adenovirus type 11 (HAdV-11) strain QS, isolated in China, was conducted, and its sequence was compared with the sequences of strains within the species of HAdVs. The HAdV-11 QS genome contains 34,755 nucleotides. Similar to the other HAdV subgenus B sequences, the HAdV-11 QS genome coded 37 functional proteins and could be divided into four early, two intermediate, and five late transcription regions. The amino acid sequences of the fiber and the hypervariable regions (HVRs) within the hexon gene of HAdV-11 QS were identical to the corresponding sequences of the HAdV-11a strain; further analyses that compared those amino acid sequences with the amino acid sequences of the HAdV species subgenus B:2 strains revealed that the highest degree of homology (>99.2%) existed between HAdV-11 QS and the prototypical HAdV-14 strain, except for a few coding sequences of HVRs within the hexon gene, DNA polymerase, pVI, and pre-terminal protein. This indicate that HAdV-11 strain QS, isolated in China, is a recombinant adenovirus of HAdV-14, and the recombination analyses also confirmed this finding. It is difficult to clarify the time and manner of the recombination, and further investigations are required to determine whether the emergence of recombination between HAdV-11a and HAdV-14 might increase virulence, thereby posing a new global challenge with regard to acute respiratory diseases in the near future.

* Corresponding author. Mailing address: State Key Laboratory for Molecular Virology & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 27, Nanwei Road, Xuanwu District, Beijing 100050, People's Republic of China. Phone and fax: 0086-10-63028480. E-mail: wenbo_xu1{at}yahoo.com.cn

{triangledown} Published ahead of print on 12 August 2009.

{dagger} These authors have contributed equally to this study and are the first authors.

Journal of Clinical Microbiology, October 2009, p. 3082-3090, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00282-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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