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Journal of Clinical Microbiology, October 2009, p. 3147-3155, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00900-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Brucella Genotypes in Patients at a Major Hospital in Central Peru{triangledown}

Karsten Nöckler,1 Ryan Maves,2 David Cepeda,2 Angelika Draeger,1 Anne Mayer-Scholl,1 Jesus Chacaltana,3 María Castañeda,3 Benjamin Espinosa,2 Rosa Castillo,2 Eric Hall,2 Sascha Al Dahouk,4 Robert H. Gilman,5 Franco Cabeza,6 and Henk L. Smits7*

Federal Institute for Risk Assessment, Berlin, Germany,1 United States Naval Medical Research Center Detachment, Lima, Peru,2 Hospital Nacional Daniel Alcides Carrión, Callao, Peru,3 RWTH Aachen University, Aachen, Germany,4 Johns Hopkins University, Baltimore, Maryland,5 Asociación Benéfica Proyectos en Informática, Salud, Medicina y Agricultura (A.B. PRISMA), Lima, Peru,6 Royal Tropical Institute/Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands7

Received 7 May 2009/ Returned for modification 29 June 2009/ Accepted 22 July 2009

The multiple-locus variable-number repeat analysis of 90 human Brucella melitensis isolates from a large urban area in central Peru revealed variations at 4 (Bruce07, Bruce09, Bruce18, and Bruce42) out of 16 loci investigated, of which 1 (Bruce42) also is used for species identification. Ten genotypes were identified, separated by the number of Bruce42 repeats into two groups that may have distinct phenotypic characteristics. Whereas genotypes with five or six Bruce42 repeats were cultured mainly from adult patients, genotypes with three Bruce42 repeats were isolated from children and young adolescents as well as from adults. In addition, the isolates with three Bruce42 repeats were obtained more often from patients with splenomegaly (P = 0.02) or hepatomegaly (P = 0.006). An annual variation in the diversity of genotypes was observed, possibly reflecting changes in sources of fresh dairy products, supply routes to city shops and markets, and the movement of infected dairy goat herds.

* Corresponding author. Mailing address: KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen (KIT), Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. Phone: 31 (0)20 5665470. Fax: 31 (0)20 6971841. E-mail: h.smits{at}kit.nl

{triangledown} Published ahead of print on 5 August 2009.

Journal of Clinical Microbiology, October 2009, p. 3147-3155, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00900-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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