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Journal of Clinical Microbiology, October 2009, p. 3156-3160, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00510-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Highly Sensitive Fetal Goat Tongue Cell Line for Detection and Isolation of Foot-and-Mouth Disease Virus {triangledown}

K. E. Brehm,1* N. P. Ferris,2 M. Lenk,3 R. Riebe,3 and B. Haas1

Friedrich-Loeffler-Institute, Institute of Diagnostic Virology, Südufer 10, 17493 Greifswald-Insel Riems, Germany,1 Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom,2 Friedrich-Loeffler-Institute, Institute of Infectology, Südufer 10, 17493 Greifswald-Insel Riems, Germany3

Received 12 March 2009/ Returned for modification 17 April 2009/ Accepted 29 July 2009

A fetal goat cell line (ZZ-R 127) supplied by the Collection of Cell Lines in Veterinary Medicine of the Friedrich Loeffler Institute was examined for susceptibility to infection by foot-and-mouth disease (FMD) virus (FMDV) and by two other viruses causing clinically indistinguishable vesicular conditions, namely, the viruses of swine vesicular disease and vesicular stomatitis. Primary bovine thyroid (BTY) cells are generally the most sensitive cell culture system for FMDV detection but are problematic to produce, particularly for laboratories that infrequently perform FMD diagnostic tests and for those in countries where FMD is endemic that face problems in sourcing thyroid glands from FMD-negative calves. Strains representing all seven serotypes of FMDV could be isolated in ZZ-R 127 cells with a sensitivity that was considerably higher than that of established cell lines and within 0.5 log of that for BTY cells. The ZZ-R 127 cell line was found to be a sensitive, rapid, and convenient tool for the isolation of FMDV and a useful alternative to BTY cells for FMD diagnosis.

* Corresponding author. Mailing address: Friedrich-Loeffler-Institute, Institute of Diagnostic Virology, Südufer 10, 17493 Greifswald-Insel Riems, Germany. Phone: 49 38351 7 164. Fax: 49 38351 7 226. E-mail: katharina.brehm{at}fli.bund.de

{triangledown} Published ahead of print on 5 August 2009.

Journal of Clinical Microbiology, October 2009, p. 3156-3160, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00510-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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