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Journal of Clinical Microbiology, October 2009, p. 3294-3300, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00355-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genetic Evolution of H9 Subtype Influenza Viruses from Live Poultry Markets in Shanghai, China {triangledown}

Fei-Fei Ge, Jin-Ping Zhou, Jian Liu, Jian Wang, Wei-Yi Zhang, Li-Ping Sheng, Feng Xu, Hou-Bing Ju, Quan-Yun Sun, and Pei-Hong Liu*

Shanghai Animal Disease Control Center, Shanghai 201103, People's Republic of China

Received 17 February 2009/ Returned for modification 21 May 2009/ Accepted 22 July 2009

H9N2 influenza viruses have become established and maintain long-term endemicity in poultry. The complete genomes of seven avian H9N2 influenza viruses were characterized. These seven influenza virus isolates were obtained from live poultry markets in Shanghai, China, in 2002 and from 2006 to 2008. Genetic analysis revealed that all seven isolates had an RSSR motif at the cleavage site of hemagglutinin (HA), indicating low pathogenicity in chickens. Phylogenetic analyses indicated that the seven avian H9N2 viruses belonged to the lineage represented by Duck/Hong Kong/Y280/97 (H9N2), a virus belonging to the Chicken/Beijing/1/94-like (H9N2) lineage, and that they are all quadruple reassortants consisting of genes from different lineages. The six internal genes of the isolates possessed H5N1-like sequences, indicating that they were reassortants of H9 and H5 viruses. All of the viruses had nonstructural (as well as HA and neuraminidase) genes derived from the Duck/Hong Kong/Y280/97-like virus lineage but also had other genes of mixed avian virus origin, including genes similar to those of H5N1 viruses (Gs/GD-like). The infected chickens showed no signs of disease. These results show the genetic and biological diversity of H9N2 viruses in Shanghai and support their potential role as pandemic influenza agents.

* Corresponding author. Mailing address: Shanghai Animal Disease Control Center, Lane 855, No. 30, Hongjing Road, Shanghai 201103, China. Phone: 86-21-62693187. Fax: 86-21-62696318. E-mail: cathrinevet{at}yahoo.com.cn

{triangledown} Published ahead of print on 5 August 2009.

Journal of Clinical Microbiology, October 2009, p. 3294-3300, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00355-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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