This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Castle, P. E.
Right arrow Articles by Kornegay, J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Castle, P. E.
Right arrow Articles by Kornegay, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2009, p. 3344-3347, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00725-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of a Prototype Real-Time PCR Assay for Carcinogenic Human Papillomavirus (HPV) Detection and Simultaneous HPV Genotype 16 (HPV16) and HPV18 Genotyping{triangledown}

Philip E. Castle,1* Mark Sadorra,2 Tiffany Lau,2 Carrie Aldrich,2 Francisco A. R. Garcia,3 and Janet Kornegay2

Division of Cancer Epidemiology, National Cancer Institute, NIH, DHHS, Bethesda, Maryland,1 Roche Molecular Systems, Alameda, California,2 University of Arizona Health Sciences Center, Tucson, Arizona3

Received 9 April 2009/ Returned for modification 12 June 2009/ Accepted 4 August 2009

Results from a prototype real-time PCR assay that separately detected human papillomavirus genotype 16 (HPV16), HPV18, and 12 other carcinogenic HPV genotypes in aggregate (cobas 4800 HPV test) and results from a PCR assay that detects 37 HPV genotypes individually (Linear Array) were compared using a convenience sample of cervical specimens (n = 531). The percentage of total agreement between the two assays was 94.7% (95% confidence interval, 92.5 to 96.5%). The Linear Array test was more likely than cobas 4800 HPV test to test positive for the 12 other carcinogenic HPV genotypes among women without evidence of cervical disease (P = 0.004).

* Corresponding author. Mailing address: Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Blvd., Room 5030, MSC 7234, Bethesda, MD 20892-7234. Phone: (301) 435-3976. Fax: (301) 402-0916. E-mail: castlep{at}mail.nih.gov

{triangledown} Published ahead of print on 12 August 2009.

Journal of Clinical Microbiology, October 2009, p. 3344-3347, Vol. 47, No. 10
0095-1137/09/$08.00+0     doi:10.1128/JCM.00725-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»