This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Pietsch, C.
Right arrow Articles by Liebert, U. G.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pietsch, C.
Right arrow Articles by Liebert, U. G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2009, p. 3569-3576, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.01471-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Molecular Characteristics of German G8P[4] Rotavirus Strain GER1H-09 Suggest that a Genotyping and Subclassification Update Is Required for G8{triangledown}

C. Pietsch,1* L. Petersen,2 L. Patzer,2 and U. G. Liebert1

Institute of Virology, Leipzig University, Leipzig, Germany,1 Department of Pediatrics, Hospital St. Elisabeth and St. Barbara, Halle/Saale, Germany2

Received 30 July 2009/ Returned for modification 20 August 2009/ Accepted 31 August 2009

A rare G8P[4] rotavirus, designated GER1H-09, was detected in a stool sample from an infant suffering from repeated episodes of emesis for 2 days without diarrhea. Sequencing of all genomic RNA segments was performed, and complete coding sequences were determined. The VP7 amino acid sequence revealed a close phylogenetic relationship to human G8P[6] and G8P[8] isolates from Slovenia and Africa. GER1H-09 shared typical amino acid residues within variable regions VR3 to VR7 with those strains, and their subclassification as lineage G8-II rotaviruses is proposed. The variability in VR3 was identified as the likely reason for the failure in genotyping G8-II rotaviruses by commonly used multiplex PCR. Furthermore, the sequences of associated structural and nonstructural proteins showed high amino acid identities to DS-1-like rotaviruses. The genotype composition of GER1H-09 (G8-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) suggests the occurrence of reassortment events between G8 genotypes and human DS-1-like G2P[4] rotaviruses.

* Corresponding author. Mailing address: Institute of Virology, Leipzig University, Johannisallee 30, 04103 Leipzig, Germany. Phone: 49 3419714333. Fax: 49 3419714309. E-mail: corinna.pietsch{at}medizin.uni-leipzig.de

{triangledown} Published ahead of print on 9 September 2009.

Journal of Clinical Microbiology, November 2009, p. 3569-3576, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.01471-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»