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Journal of Clinical Microbiology, November 2009, p. 3593-3599, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00967-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Endemic and Epidemic Acinetobacter Species in a University Hospital: an 8-Year Survey{triangledown}

P. J. van den Broek,1* T. J. K. van der Reijden,1 E. van Strijen,1 A. V. Helmig-Schurter,1 A. T. Bernards,2 and L. Dijkshoorn1

Department of Infectious Diseases, Leiden University Medical Centre, Leiden, The Netherlands,1 Department of Medical Microbiology, Leiden University Medical Centre, Leiden, The Netherlands2

Received 15 May 2009/ Returned for modification 13 July 2009/ Accepted 18 September 2009

The prevalence of the currently known Acinetobacter species and related trends of antimicrobial resistance in a Dutch university hospital were studied. Between 1999 and 2006, Acinetobacter isolates from clinical samples were collected prospectively. Isolates were analyzed by amplified fragment length polymorphism fingerprinting. For species identification, a profile similarity cutoff level of 50% was used, and for strain identification, a cutoff level of 90% was used. Susceptibility for antimicrobial agents was tested by disk diffusion by following the CLSI guideline.

The incidences of Acinetobacter isolates ranged from 1.7 to 3.7 per 10,000 patients per year, without a trend of increase, during the study years. Twenty different species were distinguished. Acinetobacter baumannii (27%) and Acinetobacter genomic species (gen. sp.) 3 (26%) were the most prevalent. Other species seen relatively frequently were Acinetobacter lwoffii (11%), Acinetobacter ursingii (4%), Acinetobacter johnsonii (4%), and Acinetobacter junii (3%). One large cluster of A. baumannii, involving 31 patients, and 16 smaller clusters of various species, involving in total 39 patients, with at most 5 patients in 1 cluster, occurred. Overall, 37% of the A. baumannii isolates were fully susceptible to the tested antibiotics. There was a borderline significant (P = 0.059) trend of decreasing susceptibility. A. baumannii was the Acinetobacter species causing the largest burden of multiple-antibiotic resistance and transmissions in the hospital.

* Corresponding author. Mailing address: Department of Infectious Diseases, C5-P, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: 0031715262290. Fax: 0031715266758. E-mail: p.j.van_den_broek{at}lumc.nl

{triangledown} Published ahead of print on 30 September 2009.

Journal of Clinical Microbiology, November 2009, p. 3593-3599, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00967-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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