This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Huang, H.
Right arrow Articles by Nord, C. E.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, H.
Right arrow Articles by Nord, C. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2009, p. 3729-3731, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.01280-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of a Commercial Multiplex Real-Time PCR to the Cell Cytotoxicity Neutralization Assay for Diagnosis of Clostridium difficile Infections{triangledown}

Haihui Huang,1,2 Andrej Weintraub,2 Hong Fang,2 and Carl Erik Nord2*

Institute of Antibiotics, Huashan Hospital, Fudan University, 12 Wulumuqi Zhong Road, Shanghai 200040, China,1 Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, SE-141 86 Stockholm, Sweden2

Received 30 June 2009/ Returned for modification 17 August 2009/ Accepted 31 August 2009

A commercial multiplex real-time PCR assay (Cepheid Xpert C. difficile assay) for the diagnosis of Clostridium difficile infection was evaluated. The sensitivity and specificity of the Cepheid assay were 97.1% and 93.0% for fresh stools, using the cell cytotoxicity neutralization assay as the reference. Using PCR ribotyping as the reference for ribotype 027 strains, the corresponding figures were 100% and 98.1%, respectively.

* Corresponding author. Mailing address: Division of Clinical Microbiology, Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. Phone: 46 8 585 878 38. Fax: 46 8 585 879 33. E-mail: carl.erik.nord{at}ki.se

{triangledown} Published ahead of print on 9 September 2009.

Journal of Clinical Microbiology, November 2009, p. 3729-3731, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.01280-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»