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Journal of Clinical Microbiology, November 2009, p. 3732-3734, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00921-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

High Interlaboratory Reproducibility of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Species Identification of Nonfermenting Bacteria{triangledown}

A. Mellmann,1* F. Bimet,2 C. Bizet,2 A. D. Borovskaya,3 R. R. Drake,4 U. Eigner,5 A. M. Fahr,5 Y. He,6,{dagger} E. N. Ilina,3 M. Kostrzewa,7 T. Maier,7 L. Mancinelli,8 W. Moussaoui,9 G. Prévost,9 L. Putignani,8 C. L. Seachord,4 Y. W. Tang,6 and D. Harmsen10

Institute of Hygiene, University Hospital Muenster, D-48149 Muenster, Germany,1 Centre de Ressources Biologiques de l'Institut Pasteur, Department of Microbiology, Institut Pasteur, F-75724 Paris, France,2 Research Institute of Physical-Chemical Medicine, 119992 Moscow, Russia,3 Department of Microbiology and Molecular Cell Biology, Center for Biomedical Proteomics, Eastern Virginia Medical School, Norfolk, Virginia,4 Labor Limbach, 69126 Heidelberg, Germany,5 Departments of Medicine and Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232,6 Bruker Daltonik, GmbH, Bremen, Germany,7 Microbiology Unit, Bambino Gesù Children's Hospital, Health Care and Research Institute, 00165 Rome, Italy,8 Institut de Bactériologie UR Physiopathologie et Médicine Translationnelle, F-67000 Strasbourg, France,9 Department for Periodontology, University Hospital Muenster, D-48149 Muenster, Germany,10

Received 11 May 2009/ Returned for modification 29 June 2009/ Accepted 15 September 2009

Matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (using eight laboratories) achieved 98.75% interlaboratory reproducibility. Only 6 of the 480 samples were misidentified due to interchanges (4 samples) or contamination (1 sample) or not identified because of insufficient signal intensity (1 sample).

* Corresponding author. Mailing address: Institut für Hygiene, Universitätsklinikum Münster, Robert-Koch-Str. 41, D-48149 Münster, Germany. Phone: 49-251-83 52136. Fax: 49-251-83 55688. E-mail: mellmann{at}uni-muenster.de

{triangledown} Published ahead of print on 23 September 2009.

{dagger} Present address: Futian Hospital, Guangdong Medical College, Shenzhen, China.

Journal of Clinical Microbiology, November 2009, p. 3732-3734, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00921-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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