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Journal of Clinical Microbiology, November 2009, p. 3747-3748, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.01391-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Failure of the BD GeneOhm StaphSR Assay for Direct Detection of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates in Positive Blood Cultures Collected in the United States {triangledown}

James W. Snyder,1* Gina K. Munier,2 Stacy A. Heckman,2 Pamela Camp,3 and Timothy L. Overman3,4

Department of Pathology & Laboratory Medicine, University of Louisville, Louisville, Kentucky 40202-8204,1 University of Louisville Hospital, Louisville, Kentucky 40202,2 Pathology and Laboratory Medicine Service, Veterans Affairs Medical Center, Lexington, Kentucky 40502-2236,3 Department of Pathology & Laboratory Medicine, University of Kentucky, Lexington, Kentucky 40536-02984

Received 16 July 2009/ Returned for modification 24 August 2009/ Accepted 17 September 2009

Fifty-nine Staphylococcus aureus isolates from one hundred blood cultures containing gram-positive cocci in clusters were identified by conventional methods and the BD GeneOhm StaphSR assay (SR). The SR misidentified three methicillin (meticillin)-resistant S. aureus (MRSA) isolates as methicillin-susceptible S. aureus (MSSA), while one MSSA isolate tested negative for S. aureus. The three MRSA isolates were strains with MREJ types that cannot be detected by the currently available SR.

* Corresponding author. Mailing address: Department of Pathology & Laboratory Medicine, University of Louisville, 530 South Jackson Street, Louisville, KY 40202-8204. Phone: (502) 852-1777. Fax: (502) 852-1771. E-mail: jwsnyd01{at}louisville.edu

{triangledown} Published ahead of print on 30 September 2009.

Journal of Clinical Microbiology, November 2009, p. 3747-3748, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.01391-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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