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Journal of Clinical Microbiology, November 2009, p. 3753-3755, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.01497-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparative Evaluation of a Commercially Available Automated System for Extraction of Viral DNA from Whole Blood: Application to Monitoring of Epstein-Barr Virus and Cytomegalovirus Load {triangledown}

Sylvie Pillet, Thomas Bourlet, and Bruno Pozzetto*

Laboratory of Bacteriology-Virology-Hygiene, University Hospital of Saint-Etienne, 42055 Saint-Etienne Cedex 02, France

Received 3 August 2009/ Accepted 19 August 2009

The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% for EBV and 87.2% for CMV.

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Virologie-Hygiène, Hôpital Nord, Centre Hospitalier Universitaire de Saint-Etienne, F-42055 Saint-Etienne Cedex 02, France. Phone: 33 4 77 82 84 34. Fax: 33 4 77 82 84 60. E-mail: Bruno.pozzetto{at}univ-st-etienne.fr

{triangledown} Published ahead of print on 26 August 2009.

Journal of Clinical Microbiology, November 2009, p. 3753-3755, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.01497-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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