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Journal of Clinical Microbiology, November 2009, p. 3765-3768, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00934-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Two Distinct Clones of Methicillin-Resistant Staphylococcus aureus (MRSA) with the Same USA300 Pulsed-Field Gel Electrophoresis Profile: a Potential Pitfall for Identification of USA300 Community-Associated MRSA{triangledown}

Anders Rhod Larsen,1,{dagger} Richard Goering,2 Marc Stegger,1 Jodi A. Lindsay,3 Katherine A. Gould,3 Jason Hinds,3 Marit Sørum,1 Henrik Westh,4,5 Kit Boye,4 and Robert Skov1*

National Center for Antimicrobials and Infection Control, Statens Serum Institut, Copenhagen, Denmark,1 Department of Microbiology, Saint Joseph Hospital, Creighton University Medical Centre, Omaha, Nebraska,2 Centre for Infection, Division of Cellular & Molecular Medicine, St. George's, University of London, London, United Kingdom,3 Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark,4 Faculty of Health, University of Copenhagen, Copenhagen, Denmark5

Received 12 May 2009/ Returned for modification 17 June 2009/ Accepted 8 September 2009

Analysis of methicillin-resistant Staphylococcus aureus (MRSA) characterized as USA300 by pulsed-field gel electrophoresis identified two distinct clones. One was similar to community-associated USA300 MRSA (ST8-IVa, t008, and Panton-Valentine leukocidin positive). The second (ST8-IVa, t024, and PVL negative) had different molecular characteristics and epidemiology, suggesting independent evolution. We recommend spa typing and/or PCR to discriminate between the two clones.

* Corresponding author. Mailing address: National Center for Antimicrobials and Infection Control, Statens Serum Institut, B.47, Artillerivej 5, DK-2300 Copenhagen. Phone: 45 3268 8348. Fax: 45 3268 3231. E-mail: rsk{at}ssi.dk

{triangledown} Published ahead of print on 16 September 2009.

{dagger} Present affiliation: LEO Pharma, Industriparken 55, DK-2750 Ballerup, Denmark.

Journal of Clinical Microbiology, November 2009, p. 3765-3768, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00934-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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