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Journal of Clinical Microbiology, November 2009, p. 3769-3772, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00303-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of the Xpert Methicillin-Resistant Staphylococcus aureus (MRSA) Assay, BD GeneOhm MRSA Assay, and Culture for Detection of Nasal and Cutaneous Groin Colonization by MRSA{triangledown}

P. G. Kelley,1,2 E. A. Grabsch,1 B. P. Howden,1,2* W. Gao,1 and M. L. Grayson1,2,3,4*

Infectious Diseases,1 Microbiology Departments, Austin Health, Heidelberg, Victoria,2 Department of Epidemiology and Preventive Medicine, Monash University, Clayton, Victoria,3 Department of Medicine, University of Melbourne, Parkville, Victoria, Australia4

Received 10 February 2009/ Returned for modification 6 May 2009/ Accepted 20 August 2009

Detection of methicillin (meticillin)-resistant Staphylococcus aureus colonization was assessed using combined nose and groin swabs in two commercial PCR assays (the Xpert MRSA assay and the BD GeneOhm MRSA assay). Compared to routine culture, both had similar sensitivities (87.0% versus 84.8%, respectively) and specificities (93.8% versus 92.7%, respectively). Combined PCR assays provide a rapid and more-complete assessment of colonization at a cost similar to that of single-site analysis.

* Corresponding author. Mailing address: Infectious Diseases Department, Austin Health, Studley Rd., Heidelberg, Victoria, Australia 3084. Phone: (61-3) 9496-6676. Fax: (61-3) 9496-6677. E-mail for M. L. Grayson: Lindsay.Grayson{at}austin.org.au. E-mail for B. P. Howden: Benjamin.Howden{at}austin.org.au

{triangledown} Published ahead of print on 26 August 2009.

Journal of Clinical Microbiology, November 2009, p. 3769-3772, Vol. 47, No. 11
0095-1137/09/$08.00+0     doi:10.1128/JCM.00303-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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