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Journal of Clinical Microbiology, February 2009, p. 410-420, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01432-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genomics-Based Molecular Epidemiology of Campylobacter jejuni Isolates from Feedlot Cattle and from People in Alberta, Canada{triangledown} ,{dagger}

Sherry J. Hannon,1* Eduardo N. Taboada,2 Margaret L. Russell,3 Brenda Allan,4 Cheryl Waldner,1 Heather L. Wilson,4 Andrew Potter,4 Lorne Babiuk,4,{ddagger} and Hugh G. G. Townsend1,4

Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada S7N 5B4,1 Laboratory for Foodborne Zoonoses (Lethbridge Laboratory), Public Health Agency of Canada, c/o Animal Diseases Research Institute, P.O. Box 640, Township Road 9-1, Lethbridge, Alberta, Canada T1J 3Z4,2 Department of Community Health Sciences, Faculty of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1,3 Vaccine and Infectious Disease Organization, 120 Veterinary Road, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E34

Received 25 July 2008/ Returned for modification 14 September 2008/ Accepted 15 November 2008

Feedlot cattle in Alberta, Canada, have been identified as reservoirs for Campylobacter jejuni, an important human pathogen. Oligonucleotide DNA microarrays were used as a platform to compare C. jejuni isolates from feedlot cattle and human clinical cases from Alberta. Comparative genomic hybridization (CGH) analysis was performed on 87 isolates (46 bovine, 41 human) obtained within the same geographical regions and time frame. Thirteen CGH clusters were obtained based on overall comparative genomic profile similarity. Nine CGH clusters contained human and cattle isolates, three contained only human isolates, and one contained only cattle isolates. The study isolates clustered regardless of temporal or geographical frameworks. In addition, array genes (n = 1,399) were investigated on a gene-by-gene basis to see if any were unequally distributed between human and cattle sources or between clusters dominated by either human or cattle isolates ("human enriched" versus "cattle enriched"). Using Fisher's exact test with the Westfall and Young correction for these comparisons, a small number of differentially distributed genes were identified. Our findings suggest that feedlot cattle and human C. jejuni strains are very similar and may be endemic within Alberta. Further, the common distribution of human clinical and bovine C. jejuni isolates within the same genetically based clusters suggests that dynamic and important transmission routes between cattle and human populations may exist.

* Corresponding author. Present address: Feedlot Health Management Services Ltd., P.O. Box 140, Okotoks, Alberta, Canada T2S 2A2. E-mail: sherryh{at}feedlothealth.com

{triangledown} Published ahead of print on 26 November 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{ddagger} Present address: University of Alberta, 3-7 University Hall, Edmonton, Alberta, Canada T6G 2J9.

Journal of Clinical Microbiology, February 2009, p. 410-420, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01432-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



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