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Journal of Clinical Microbiology, February 2009, p. 459-462, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01181-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Infants by Use of Dried Blood Spots and an Ultrasensitive p24 Antigen Assay{triangledown}

Ada Cachafeiro,1 Gayle G. Sherman,2 Annette H. Sohn,3 Consuelo Beck-Sague,4 and Susan A. Fiscus1*

Department of Microbiology & Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina,1 Department of Molecular Medicine and Haematology, University of Witwatersrand, Johannesburg, South Africa,2 Division of Pediatric Infectious Diseases, University of California, San Francisco, San Francisco, California,3 Clínica de Familia MIR, La Romana, Dominican Republic4

Received 22 June 2008/ Returned for modification 27 September 2008/ Accepted 3 December 2008

We tested 617 dried blood spots (DBS) from human immunodeficiency virus-exposed infants from five countries using an ultrasensitive p24 antigen assay (Up24). The sensitivity was 94.4% (67/71) and the specificity was 100% (431/431) for infants with DBS specimens ≤20 months old; DBS older than 30 months demonstrated only 72.2% sensitivity (39/54) (P < 0.001) but displayed 100% specificity (61/61).

* Corresponding author. Mailing address: Department of Microbiology & Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7290. Phone: (919) 966-6872. Fax: (919) 966-9873. E-mail: fiscussa{at}med.unc.edu

{triangledown} Published ahead of print on 10 December 2008.

Journal of Clinical Microbiology, February 2009, p. 459-462, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01181-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Finnegan, J., Nobel, K.-A., Lodha, R. (2009). Evidence behind the WHO Guidelines: Hospital Care for Children: What is the Role of HIV Antigen Testing in Infants <12-months Old?. J Trop Pediatr 55: 216-218 [Full Text]  


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